GATE BT Core MSQ MCQs

A. A thermostable DNA polymerase is used. B. Primers define the amplified region. C. Denaturation separates DNA strands. D. Translation occurs during each PCR cycle.

A. Vmax increases with enzyme concentration under otherwise fixed conditions. B. Km has concentration units in Michaelis–Menten kinetics. C. A competitive inhibitor can increase apparent Km. D. Every inhibitor permanently destroys the enzyme.

A. During exponential growth, cell number rises geometrically. B. Generation time is the time for a population to double. C. A chemostat can maintain steady-state growth. D. Sterilization means merely reducing microbial load slightly.